The newest SD region of O. luzonensis maps ranging from eyeless and 171M23F to the LG 12 (Tanaka mais aussi al. 2007). I did further linkage research and you will acquired a few men recombinants having this region. One to men had a good recombination breakpoint ranging from OluX2-8 and you can OluX2-twenty five, plus the most other got an effective breakpoint anywhere between OluX3-34 and you can OluX4-six, refining new SD area so you can ranging from OluX2-8 and you will OluX4-6 ( Figure 1A ).

(A) Hereditary and you may bodily map of gender-deciding regions to your X and you may Y chromosomes. 1, typical XY men; 2, XX lady; step 3 and you will 4, recombinant men. Bluish line, places produced from the brand new Y chromosome; red, on X-chromosome. Red-bar, BAC clones; blue pub, fosmid clones. Black arrows suggest predicted genes. (B) RT–PCR circumstances off PGs (PG1–9) regarding the XX and you will XY muscles trunk from the 0 days immediately after hatching. Meters, size marker.

I constructed a good BAC library regarding a keen XX seafood and an effective fosmid library out-of a great YY fish, and in addition we generated real maps of SD region of the X and you will Y chromosomes. This place are wrapped in one or two BAC clones (OluBXKN2 and you may OluBXKN1) toward X chromosome in accordance with 7 fosmid clones (OluFYstep one3-1, OluFY24-step one, OluFY18-1, OluFY3-step 1, OluFY8-1, OluFY7-step one, and you will OluFY29-1) into Y-chromosome ( Contour 1A ). The whole nucleotide succession is influenced by having fun with shotgun sequencing, with the exception of a repeated region inside the OluFY3-step one and OluBXKN1. Restrict data regarding each other clones exhibited the duration of brand new repeated area is actually a similar for both chromosomes (analysis not revealed). (lebih…)